Endogenous Ceramide Contributes to the Transcytosis of oxLDL across Endothelial Cells and Promotes Its Subendothelial Retention in Vascular Wall
Figure 3
Fluorescence microscopic analysis of FITC-oxLDL uptake in HUVECs. HUVECs cultured on coverslips were pretreated with 30 μM MYR, 10 μM DES, 30 μM D609, or 10 μM NOE for 12 h, and oxLDL (50 μg/mL) was added to incubate for 3 h. (a) Representative fluorescence microscopic images of FITC-oxLDL uptake of HUVECs. Scale bars are equal to 50 μm. “Naïve oxLDL” group was incubated with unlabeled oxLDL. (b) Quantification analysis of FITC-oxLDL uptake in HUVECs. versus control, .
