Taurine Rescues Cisplatin-Induced Muscle Atrophy In Vitro: A Morphological Study
Figure 4
TEM micrographs of C2C12 myotubes after DMSO (a), starvation (b)-(c), CisPt 50 μM at 4 h (d)–(g), and 24 h (h)-(i) exposure. Note elongated DMSO exposed controls (a), but at 4 h exposure to CisPt perinuclear autophagic vacuoles (d): at higher magnification, in square 1, in the same myotube such structures resembled autophagosomes (arrowhead) and autophagolysosomes (arrow) (e)-(f), and, in square 2, enlarged endoplasmic reticulum (g). Under 50 μM CisPt for 24 h clusters of autophagolysosomes (arrow) near the sarcolemma (h-i). In starved myotubes, irregular electron dense deposits (b) that at higher magnification (squared area) resembled secondary lysosomes and osmiophilic membranous debris (c). (a), (b), (e), and (h) ; (c), (f), (g), and (i) .
