Electrochemically Reduced Water Protects Neural Cells from Oxidative Damage

Figure 1

Suppressive effect of ERW against H₂O₂-induced cytotoxicity on PC12 and SFME cells. Viable cell numbers were measured using a WST-8 kit. (a) Pheochromocytoma PC12 cells were cultured in FBS/HS/DMEM medium with or without ERW and the number of surviving cells were set as controls. H₂O₂ (100, 200 and 500 μM) was added to the control medium and it was cultured for 24 h. Viable cells were measured at 450 nm using a microtiter plate reader. (b) Serum-free mouse embryo (SFME) cells were cultured in a 1 : 1 mixture of DMEM : Ham’s F12 medium with or without ERW and the number of surviving cells were set as controls. To the control medium, H₂O₂ (50, 100 and 200 μM) was added and it was cultured for 24 h. Viable cells were measured at 450 nm using a microtiter plate reader. Data are presented as mean ± standard deviation (SD) for three independent experiments ( each set). and .

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