Electrochemically Reduced Water Protects Neural Cells from Oxidative Damage

Figure 5

H₂O₂-induced ROS scavenging ability of ERW on N1E115 cells. Differentiated N1E115 cells were treated for 10 min with ERW with or without 200 μM H₂O₂ and intracellular H₂O₂ levels were detected with a DCFH-DA probe and measured using a confocal laser microscope. Representative photographs of three independent experiments are shown: (a) MQ control, (b) ERW, (c) MQ + 100 μM H₂O₂, and (d) ERW + 100 μM H₂O₂. (e) Photographs were used to calculate intracellular fluorescence intensities reflecting H₂O₂ levels in the cells for each treatment. Data are expressed as mean ± standard deviation (SD) for three independent experiments. and .

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