Electrochemically Reduced Water Protects Neural Cells from Oxidative Damage

Figure 6

Suppressive effect of ERW on SNP-induced NO toxicity in PC12 cells. Cells were cultured for 24 h with the medium containing ERW or MQ-water each supplemented with 0, 200, 400, and 600 μM SNP. (a) Cell viability was measured by a WST-8 kit. Data are expressed as mean ± standard deviation (SD) for three independent experiments. . (b) ERW was assessed for its intracellular NO scavenging ability using the DAR-4M AM bioimaging probe. Representative photographs of NO dependent fluorescence levels incubated with MQ, ERW, and 200 μM SNP added-MQ and ERW are shown. (c) Fluorescence intensities of 50 cells treated with MQ or ERW containing 200 μM SNP were measured and Tukey’s multiple comparison method was used to compare the effect of MQ and ERW.

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