Anandamide Protects HT22 Cells Exposed to Hydrogen Peroxide by Inhibiting CB1 Receptor-Mediated Type 2 NADPH Oxidase
Figure 6
AEA decreased intracellular ROS generation via CB1. The intracellular ROS levels were assessed by ROS reagent kit. (a)–(e) indicate the fluorescence intensity of ROS. (a) Control cells cultured in drug-free medium. (b) Cells exposed to 200 μM H2O2 for 3 h. (c) Cells exposed to 10 μM AEA plus 200 μM H2O2 for 3 h. (d) Cells exposed to 10 μM AEA plus 10 μM AM251 in the presence of 200 μM H2O2 for 3 h. (e) Cells exposed to 10 μM CB1 antagonist AM251 plus 200 μM H2O2 for 3 h. (f) Statistical results of (a)–(e). Results are expressed as means ± SD (). versus the control (no H2O2, no AEA, and no AM251), versus the cells exposed to H2O2 alone, and versus the cells exposed to AEA plus H2O2.