Toona sinensis Inhibits LPS-Induced Inflammation and Migration in Vascular Smooth Muscle Cells via Suppression of Reactive Oxygen Species and NF-B Signaling Pathway
Figure 1
Effect of TS and GA on A7r5 cell viability. (a) Rat aortic SMC (A7r5) cells were incubated with TS (0, 25, 50, 75, and 100 μg/mL) and GA (5 μg/mL) for 12 h. (b) A7r5 cells were pretreated with TS (0, 25, 50, 75, and 100 μg/mL) and GA (5 μg/mL) for 2 h and then stimulated by LPS (100 ng/mL) for 24 h. The cell cultures were harvested, and cell viability was determined using the MTT assay (200x magnification). The percentage (%) of cell viability was calculated by the following equation: (A570 of treated cells/A570 of untreated cells) × 100. The results represent the mean ± SD of three independent assays. significant difference () compared to the control or LPS-treated groups, respectively. TS, Toona sinensis; GA, gallic acid.
