Toona sinensis Inhibits LPS-Induced Inflammation and Migration in Vascular Smooth Muscle Cells via Suppression of Reactive Oxygen Species and NF-B Signaling Pathway
Figure 4
TS and GA inhibit PGE2 production through the downregulation of COX-2 protein expression in LPS-activated A7r5 cells. Cells were preincubated with or without TS (25–100 μg/mL) or GA (5 μg/mL) for 2 h and then stimulated with LPS (100 ng/mL) for 8 h. (a) PGE2 production was measured using a commercially available EIA kit, as described in the methods. (b) Protein (50 μg) from each sample was resolved by 10% SDS-PAGE, followed by western blotting. The results represent the mean ± SD of three assays. significant difference () in comparison to control or LPS-treated groups, respectively.
