Salidroside Stimulates Mitochondrial Biogenesis and Protects against H₂O₂-Induced Endothelial Dysfunction

Figure 4

Effects of SAL on H₂O₂-induced activation of eNOS, AMPK, and Akt. After pretreatment with SAL (10 μM) for 20 h, H₂O₂ (0.1 μM or 100 μM) was added for another 4 h, and the lysates were analyzed by western blot. (a) Representative immunoblots. (b, c, d) The histogram shows quantitation of eNOS-ser1177, eNOS, AMPKa-thr172, AMPK, Akt-ser473, and Akt expression. The expressions were normalized to that obtained in untreated cells. ^∗𝑃<0.05, ^∗∗𝑃<0.01 versus control; ^†𝑃<0.05 versus H₂O₂, 𝑛 = 4–6.

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