Effects of Downregulation of MicroRNA-181a on H2O2-Induced H9c2 Cell Apoptosis via the Mitochondrial Apoptotic Pathway
Figure 3
Gpx1 3′-UTR is a direct target of miR-181a. (a) Predicted miR-181a target site in the 3′-UTR of rat Gpx1. The wild-type rat Gpx1 mRNA sequence is shown with the potential binding sites. The mature miR-181a sequence and potential binding between the miR-181a seed region and the rat Gpx1 3′-UTR sequence are shown, with the mutated bases indicated above in red. (b) Rat Gpx1 3′-UTR reporter and the miR-181a binding site mutant reporter were cotransfected with either the mature miR-181a (50 nM) or miR-CTL into HEK 293T cells, followed by cell lysis and luciferase assays 48 h later. versus Gpx1-WT+miR-CTL group; the values represent the mean SEM, . (c) Gpx1 and β-actin protein levels were detected by western blotting 48 h after H9c2 cells were transfected with miR-CTL, miR-181a (50 nM), anti-CTL, or anti-miR-181a (100 nM). versus miR-CTL; versus anti-CTL group; the values represent the mean SEM; .
