Polyol Pathway Exacerbated Ischemia/Reperfusion-Induced Injury in Steatotic Liver
Figure 1
ARI normalized fatty mouse liver function and histology. (a) The hepatic TG levels in Sham, Ctrl, and ARI groups showed no differences after HFD treatment. (b) Both in H&E staining and SEM, ARI group demonstrated less hepatic necrosis, infiltration of inflammatory cells (black arrow), and ultrastructural disturbances relative to the Ctrl group, whereas Sham group showed an almost intact histological architecture; additionally, intrahepatic lipid droplets are clearly visible. (c) In Suzuki score representing the extent of hepatic necrosis and inflammation, Ctrl group was notably higher than Sham group, and this increase was markedly mitigated by ARI administration. (d)-(e) In the flow cytometry, the Ctrl group showed markedly higher proportions of apoptotic and necrotic hepatocytes than Sham group, and ARI significantly reverted these trends. (f)-(g) I/R insult dramatically elevated serum transaminases (ALT and AST) level, and ARI clearly reversed these changes (for each condition, data are expressed as mean ± SEM and analyzed by unpaired Student’s -test; , ; ). ld, lipid droplet; hn, hepatocyte nucleus; bc, bile canaliculus; ms, microvillus; sec (dsec), (damaged) sinusoidal endothelial cell; m (dm), (damaged) mitochondria; pmn, polymorphonucleocyte; rbc, red blood cell; der, dilated endoplasmic reticulum; ly, lysosome; vc, vacuole.
