Oxidative Medicine and Cellular Longevity / 2014 / Article / Fig 4

Research Article

Novel Functional Changes during Podocyte Differentiation: Increase of Oxidative Resistance and H-Ferritin Expression

Figure 4

Mature podocytes are highly resistant to oxidative stress. (a) Nondifferentiated and differentiated podocytes were exposed to H2O2 (0–500 μmol/L in HBSS) for 4 hours. Cell viability was assessed by MTT assay. (b) Nondifferentiated and differentiated podocytes were subjected to heme treatment (0–5 μmol/L in HBSS). After 1 hour heme was replaced by HBSS. Cell viability was measured by MTT assay after a 4-hour incubation period. (c) Nondifferentiated and differentiated podocytes were treated with heme (2.5 μmol/L in HBSS) or vehicle for 1 hour. After washing the heme away cells were exposed to H2O2 (125 μmol/L in HBSS) or vehicle for 4 hours. Cell viability was determined by MTT assay. Results are shown as mean values ± SEM of at least three independent experiments each performed in quadruplicate. , .
976394.fig.004a
(a)
976394.fig.004b
(b)
976394.fig.004c
(c)

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19. Sign up here as a reviewer to help fast-track new submissions.