Novel Functional Changes during Podocyte Differentiation: Increase of Oxidative Resistance and H-Ferritin Expression
Figure 4
Mature podocytes are highly resistant to oxidative stress. (a) Nondifferentiated and differentiated podocytes were exposed to H2O2 (0–500 μmol/L in HBSS) for 4 hours. Cell viability was assessed by MTT assay. (b) Nondifferentiated and differentiated podocytes were subjected to heme treatment (0–5 μmol/L in HBSS). After 1 hour heme was replaced by HBSS. Cell viability was measured by MTT assay after a 4-hour incubation period. (c) Nondifferentiated and differentiated podocytes were treated with heme (2.5 μmol/L in HBSS) or vehicle for 1 hour. After washing the heme away cells were exposed to H2O2 (125 μmol/L in HBSS) or vehicle for 4 hours. Cell viability was determined by MTT assay. Results are shown as mean values ± SEM of at least three independent experiments each performed in quadruplicate. , .
