Novel Functional Changes during Podocyte Differentiation: Increase of Oxidative Resistance and H-Ferritin Expression
Figure 7
FtH is highly expressed in differentiated podocytes. (a) Nondifferentiated and differentiated podocytes were treated with heme (0–5 μmol/L in HBSS) and harvested after 4 hours. Equal amounts of total protein (25 μg) were applied to nonreducing gel for western blotting. Western blot showed that FtH is inducible and already present in both cell types at baseline; however, basic FtH expression is more robust in differentiated cells. Induction of FtH by heme treatment is more pronounced in nondifferentiated podocytes compared to differentiated cells. (b) FtH (brown) and WT-1 (purple) staining of native human kidney section are shown. Magnification demonstrates the lumen of a glomerular capillary () and the cell body of a glomerular podocyte with its pedicles (POD) embracing the capillary wall in cross-section. Strong brown staining indicates the presence of FtH in the cytoplasm of podocytes. Scale bar: 10 μm. Representative image of 3 with similar result.
