Panaxatriol Saponins Attenuated Oxygen-Glucose Deprivation Injury in PC12 Cells via Activation of PI3K/Akt and Nrf2 Signaling Pathway
Figure 6
Role of PI3K/Akt in induction of HO-1 expression by PTS. (a) Phosphorylation of Akt induced by PTS. Cells were treated with PTS (16 μg·mL−1) for different time points as indicated. (b) Inhibition of PI3K in PTS induced phosphorylation of Akt. Cells were treated with PTS (16 μg·mL−1) in the absence and presence of 20 μM LY294002 as indicated for 6 h. (c) Inhibition of PI3K in PTS induced HO-1 expression. Cells were incubated with PTS (16 μg·mL−1) in the absence and presence of 20 μM LY294002 for 6 h. (d) Inhibition of PI3K in PTS induced Nrf2 nuclear translocation in PC12 cells. Cells were treated with PTS (16 μg·mL−1) in the absence and presence of 20 μM LY294002 as indicated for 6 h. The blots were representative of three independent experiments.
