Panaxatriol Saponins Attenuated Oxygen-Glucose Deprivation Injury in PC12 Cells via Activation of PI3K/Akt and Nrf2 Signaling Pathway
Figure 7
Increased induction of phase II enzymes by Rg1, Re, and R1 and their combination (Comb.) in PC12 cells. (a) Induction of HO-1. PC12 cells were treated for 24 h by Rg1 (2.5 μM), Re (2.5 μM), R1 (2.5 μM), and their combination, respectively. Protein was detected by Western blotting using specific antibodies against rat HO-1, whereas β-actin protein was detected in parallel as the control. (b) Induction of NQO1. Following the treatment as described in Panel (a), protein was detected by Western blotting using specific antibodies against rat NQO1 and β-actin. Relative protein expression level of HO-1 and NQO1 was normalized to that of untreated cells after correction for the expression level of β-actin. The data were reported as mean ± SD (). , , significantly different from vehicle group. The blots were representative of three independent experiments.
