Panaxatriol Saponins Attenuated Oxygen-Glucose Deprivation Injury in PC12 Cells via Activation of PI3K/Akt and Nrf2 Signaling Pathway
Figure 8
Role ofPI3K/Akt and Nrf2-Keap1 pathway in the protective effect of PTS against OGD-Rep. (a) PI3K inhibition on the protective effect of PTS against OGD-Rep. PC12 cells were treated with PTS (16 μg·mL−1) in the absence and presence of 20 μM LY294002 as indicated for 3 h, subsequently subjected to OGD-Rep treatment for 8 h, and finally maintained in oxygenated cell culture medium for another 24 h. The cell viability was determined by CCK-8 assay. (b) ARE decoy ODNs on the protective effect of PTS against OGD-Rep. Cells were directly exposed to PTS or treated with ARE decoy or mut ODNs, followed by PTS (16 μg·mL−1) treatment for 3 h. Subsequently, the cells were subjected to OGD-Rep treatment and cell viability determination as indicated above. Values represent mean ± SD (). and .
