High Potency of a Novel Resveratrol Derivative, 3,3′,4,4′-Tetrahydroxy-trans-stilbene, against Ovarian Cancer Is Associated with an Oxidative Stress-Mediated Imbalance between DNA Damage Accumulation and Repair

Figure 3

Effect of RVT and 3,3′,4,4′-THS on the generation of ROS ((a)–(c)) and the activity of SOD ((d)–(f)) and CAT ((g)–(i)) in ovarian cancer cells. The magnitude of ROS release was determined using a redox-sensitive fluorescence probe H₂DCFDA (5 μM), which was added to the medium for 1 h. The activity of the antioxidative enzymes was determined in cell lysates upon 24-h cell exposure to the stilbenes using commercially available kits. The asterisks indicate a significant difference as compared to the control group (Con). The hashes indicate a significant difference as compared to cells subjected to RVT. The values (expressed as a percentage of the control group) and signs of statistical significance located above the bars in panel (a)–(c) refer to experiments in which the cancer cells were subjected to RVT and 3,3′,4,4′-THS for 6 h. The experiments were performed in octuplicate.