Enhancement of the Acrolein-Induced Production of Reactive Oxygen Species and Lung Injury by GADD34

Figure 1

NAC prevents acrolein-induced lung injury and inflammation. Wild-type mice were intranasally instilled by 5 μmol/kg acrolein with intravenous injection of 100 μL of NAC (500 mg/kg) or equal volume of PBS. (a) Level of ROS in lung tissues was measured by DCFH-DA. (b) H&E staining of lung tissues (scale bar: 50 μm). (c) Alveolar macrophages were stained with PE-conjugated anti-CD11c and APC-conjugated anti-F4/80 and detected by FACS. (d) The expression of phospho-NF-κB p65 in the lung tissues by western blot.

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