High Throughput Screening Identifies a Novel Compound Protecting Cardiomyocytes from Doxorubicin-Induced Damage

Figure 2

Reassessment of cardiocytoprotective effect of hit compounds with a microscopy-based method. H9C2 cells were treated with the hit compounds (12 μM) for 30 min followed by a 24 h exposure to DOX (300 ng/mL). Cells were stained with Coomassie dye and photographed with a Leica MC120 HD camera connected to a Leica DM IL LED microscope (5x magnification) (a). Cell-covered area was determined with the Tscratch software (b). Mean ± SEM of 3 independent experiments was calculated (b). Only compound#10 had significant () cytoprotective effect in this assay. The structural formula of compound#10 (EODB) is shown on panel (c).

(a)

(b)

(c)