High Throughput Screening Identifies a Novel Compound Protecting Cardiomyocytes from Doxorubicin-Induced Damage

Figure 3

Characterization of the mode of DOX-induced cell death. H9C2 cells were pretreated (30 min) with compound#10 and then treated with DOX (300 ng/mL). After 24 h viability, apoptotic and necrotic cell deaths have been assessed with calcein-AM assay (a), caspase activity (b), and lactate dehydrogenase (LDH) release (c), respectively. The lead compound significantly reduced DOX-induced cytotoxicity with a stronger effect on necrosis than on the apoptotic cell death. Mean ± SEM of 3 independent experiments is presented. Stars indicate significant () DOX-induced cell death compared to control whereas hatch marks indicate significant () cytoprotection by the lead compound.

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