High Throughput Screening Identifies a Novel Compound Protecting Cardiomyocytes from Doxorubicin-Induced Damage

Figure 5

Lack of marked antioxidant effect but inhibition of DOX-induced JNK activation by EODB. We tested the radical scavenging and antioxidant effects of EODB in ABTS assay (a), in the CUPRAC assay (b), in Ampliflu Red oxidation assay (c) and in superoxide assay (d). In the concentration used in the experiments (12 μM) EODB lacked any detectable radical scavenging or antioxidant effect in the ABTS (a), CUPRAC (b) and superoxide (d) assays and displayed a small but statistically significant H₂O₂ scavenging activity (c). EODB also lacked superoxide scavenging activity (d). Trolox (12 μM) (a, b) and vitamin C (10 μM on panel (c) and 100 μM on panel (d)) were used as positive controls. Mean ± SEM of three independent experiments is presented. DOX-induced JNK activation (e) has been determined by Western blotting 24 h after DOX treatment (carried out as in the cytotoxicity experiments). ().

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