Effects of Docosahexaenoic Supplementation and In Vitro Vitamin C on the Oxidative and Inflammatory Neutrophil Response to Activation

Table 4

Effects of PMA activation, in vitro vitamin C, and DHA diet supplementation on nitrite and nitrate distribution between extracellular and intracellular neutrophil compartments.

		Control	PMA	PMA + VitC	ANOVA
					S	A	S × A
Extracellular
Nitrate (mM)	Placebo	1.07 ± 0.05	1.23 ± 0.04^#	1.03 ± 0.07^#$	X	X
	Experimental	1.16 ± 0.10	1.41 ± 0.06^*#	1.10 ± 0.07^#$
Nitrite (nM)	Placebo	458 ± 54	648 ± 48^#	422 ± 68^#$	X	X
	Experimental	545 ± 104	798 ± 65^*#	495 ± 69^#$
Intracellular
Nitrate (mM)	Placebo	572 ± 60	447 ± 45	441 ± 65		X
	Experimental	715 ± 119	494 ± 78^#	437 ± 60^#
Intracellular/extracellular relationships
Nitrate ratio	Placebo	1366 ± 213	712 ± 77^#	1305 ± 284^$		X
	Experimental	1363 ± 130	646 ± 87^#	954 ± 152^#

Statistical analysis: two-way ANOVA, . S: supplementation effect; A: activation effect; S × A: interaction between supplementation and activation effects. X indicates significant effect of the statistical factor S, A, or S × A. *Differences between placebo and experimental groups; ^#difference with respect to the control group; ^$differences between PMA and PMA + VitC group. When interaction S × A exists between supplementation and activation factors, different letters reveal significant differences. Results are the mean ± SEM.