EGCG Attenuates Uric Acid-Induced Inflammatory and Oxidative Stress Responses by Medicating the NOTCH Pathway

Figure 3

Notch signaling pathway was involved in UA-induced inflammatory reaction and oxidative stress. (a) The expression of Notch1 was confirmed by western blotting analysis in HUVEC transfected with Notch1 siRNA. (b–f) The Hes1, MCP-1, TNF-α, ICAM-1, and NF-κB mRNA were detected qRT-PCR in HUVEC with treatment as indicated. (g) Immunoblot analysis showing effects of UA with or without Notch1 inhibition by siRNA on the expression of Notch-1, IL-6, MCP-1, ICAM-1, TNF-α, Hes1, Hes5, and p-P65 in HUVEC. (h) Quantitative analysis of intracellular ROS production for the HUVEC with the indicated treatment. , .

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