Neuroprotection by Cocktails of Dietary Antioxidants under Conditions of Nerve Growth Factor Deprivation
Time-course of neuronal PC12 cells death following NGF deprivation. (a) β-III tubulin immunostaining on PC12(615) cells differentiated with NGF (10 ng/mL) for 6 days. (b) MTT assay on neuronal PC12 cells NGF-deprived for the indicated times. Data, expressed as percent of CTR-NGF at the indicated time points, are the mean ± SEM of five independent experiments, each performed in triplicate. CTR-NGF did not change at each time point. , versus CTR-NGF (ANOVA and Dunnett’s multiple comparisons test). (c) Representative images of neuronal PC12 cells, CTR, or NGF-deprived for 72 h.