Inhibition of Adenylyl Cyclase Type 5 Increases Longevity and Healthful Aging through Oxidative Stress Protection

Figure 3

SIRT1/FoxO3a/MnSOD pathway in AC5 KO, AC5 Tg, and AC6 KO. (a) and (b) AC activity at baseline and in response to forskolin stimulation was enhanced in AC5 Tg mice compared to WT. (c) AC5 regulated MnSOD expression. Downregulation of MnSOD in AC5 Tg mice hearts is shown (: 6 per group) (^∗). (d) Expression of SIRT1 by western blotting in the AC5 myocardial cells. SIRT1 was highly expressed compared to WT. (^∗). Expression of FoxO3a by western blotting in AC5 KO mouse hearts is shown. More FoxO3a was expressed in the nucleus of AC5 KO myocytes compared to WT. MnSOD expression increased in AC5 KO hearts (^∗). Data are expressed as mean ± SEM. (e) SIRT1 and MnSOD expression levels in AC6 KO did not show any difference compared to WT. Data is redrawn from Lai et al. [3].

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