Physical Training Regulates Mitochondrial Parameters and Neuroinflammatory Mechanisms in an Experimental Model of Parkinson’s Disease

Figure 2

The effects of two physical training protocols on Sirt1 levels (a and b, ) and complex I activity (c and d, ) in the striatum and hippocampus of mice exposed to 6-OHDA. Hippocampus and striatum protein extracts containing 0.2 mg total protein were separated by SDS-PAGE, transferred to nitrocellulose membranes, and probed with anti-Sirt1 antibody. Complex I activity was assayed by measuring the NADH-dependent reduction of ferricyanide. Values for Sirt1 are presented as mean area × intensity of bands ± SEM (arbitrary units), and complex I activity is expressed as mean ± SEM. , Sham versus U + 6-OHDA; , U + 6-OHDA versus training groups plus 6-OHDA. Untrained + vehicle (Sham), strength training + vehicle (STR), treadmill training + vehicle (TTR), untrained + 6-OHDA (U + 6-OHDA), strength training + 6-OHDA (STR + 6-OHDA), and treadmill training + 6-OHDA (TTR + 6-OHDA).

(a) Striatum

(b) Hippocampus

(c) Striatum

(d) Hippocampus