Metallothionein-I/II Knockout Mice Aggravate Mitochondrial Superoxide Production and Peroxiredoxin 3 Expression in Thyroid after Excessive Iodide Exposure

Figure 2

Mitochondrial superoxide production, Prx 3 protein expression, and LDH release and relative viability in the thyroid of WT and MT-I/II knockout mice following NI, 10 HI, or 100 HI diet for 14 days. (a) 100 HI intake decreased the relative viability in thyroid of MT-I/II KO and WT mice. (b) Representative western blot of Prx 3 (27 kDa), β-actin (45 kDa) was used as a loading control. Densitometric analysis showed a significant increase in Prx 3 expression in 100 HI group in the thyroid of either WT or MT-I/II KO mice, especially in MT-I/II KO mice. (c) 100 HI increased the LDH release in the thyroid of MT-I/II KO and WT mice. (d) 100 HI intake increased the mitochondrial superoxide production in thyroid of WT and MT-I/II KO mice. Histogram analysis was performed on the mean fluorescence intensity of MitoSOX Red as measured by flow cytometry. Experiments were repeated 3 times with similar results. Data are represented as mean ± SD (/group). One-way ANOVA with the LSD test was used. compared with the control group of WT or MT-I/II KO mice, respectively; ^# WT mice compared with the MT-I/II KO mice under the same treatment.

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