Generation of Adducts of 4-Hydroxy-2-nonenal with Heat Shock 60 kDa Protein 1 in Human Promyelocytic HL-60 and Monocytic THP-1 Cell Lines

Figure 2

Electron microscopic analysis of HL-60 cells treated with 10 μM HNE for 2 h. HNE-treated HL-60 cells were exposed to the anti-HNE-histidine antibody and subjected to electron microscopy and single-antibody immunogold localization as described in Section 2. Black arrows mark immune complexes between HNE-modified proteins and anti-HNE-histidine antibodies in cytosol (C), nuclei (N), and mitochondria (M).