Generation of Adducts of 4-Hydroxy-2-nonenal with Heat Shock 60 kDa Protein 1 in Human Promyelocytic HL-60 and Monocytic THP-1 Cell Lines

Figure 4

MALDI-TOF spectrum of the products of the in-gel tryptic digestion of spot number 3c from a preparative replica (200 μg protein load) of the gel shown in Figure 3(c). MALDI-TOF-MS was performed as described under Section 2. Numbers besides mass signals indicate the mass-to-charge ratios () of the multiple pseudo-molecular ions ([M⁺H⁺]) deriving from the ionization of tryptic peptides.