Oxidative Medicine and Cellular Longevity

Research Article

The Effect of Age on Osteogenic and Adipogenic Differentiation Potential of Human Adipose Derived Stromal Stem Cells (hASCs) and the Impact of Stress Factors in the Course of the Differentiation Process

Table 1

Sequences of primers used in qPCR. PPARγ: peroxisome proliferator-activated receptor gamma; COL-1: collagen type I; OPN: osteopontin; BMP-2: bone morphogenetic protein 2; ALP: alkaline phosphatase; OCN: osteocalcin; p53: tumor suppressor p53; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; bp: base pair.
GenePrimerSequence 5′-3′Amplicon length (bp)Accession number
PPARγF:AGTCCTCACAGCTGTTTGCCAAGC125XM_011533844.1
R:GAGCGGGTGAAGACTCATGTCTGTC
COL-1F:GTGATGCTGGTCCTGTTGGT123NM_000088.3
R:CACCATCGTGAGCCTTCTCT
OPNF:AAACGCCGACCAAGGTACAG213U20758.1
R:ATGCCTAGGAGGCAAAAGCAA
BMP-2F:ATGGATTCGTGGTGGAAGTG349KC294426.1
R:GTGGAGTTCAGATGATCAGC
ALPF:CGCGCTTGTGCCTGGA185XM_006710546
R:CCTGCTTTATCCCTGGAGCC
OCNF:ATGAGAGCCCTCACACTCCTC292NM_199173.4
R:CGTAGAAGCGCCGATAGGC
p53F:AGATAGCGATGGTCTGGC381NM_001126118.1
R:TTGGGCAGTGCTCGCTTAGT
GAPDHF:CATGGCCTTCCGTGTTCCTA286NM_017008.4
R:CACCACCCTGTTGCTGTAGC