L-Lactate Protects Skin Fibroblasts against Aging-Associated Mitochondrial Dysfunction via Mitohormesis

Figure 2

The effect of L-lactate on respiration and H₂O₂ production of isolated mitochondria. (a) Relative production of H₂O₂ from isolated fibroblasts mitochondria respiring on 10 mM glutamate, 1 mM malate, and 10 mM succinate measured with Amplex Red after the addition of 0–10 mM L-lactate. (b) Relative production of H₂O₂ from isolated liver mitochondria respiring on 10 mM glutamate, 1 mM malate, and 10 mM succinate measured with Amplex Red after the addition of 0–10 mM L-lactate. (c) Relative increase in production of H₂O₂ from isolated liver mitochondria without substrates (CTRL), pretreated with 10 μM rotenone (ROT), 10 μM FCCP (FCCP), or 2 mg/L oligomycin (OLIGO) measured by Amplex Red after addition of 5 mM L-lactate. (d) Increase in O₂ consumption of isolated mitochondria without substrates (CTRL), respiring on 10 mM glutamate/1 mM malate (G/M) or respiring on 10 mM succinate (S) after addition of 5 mM L-lactate. (^∗significantly different from CTRL or from 0 mM L-lactate; ).

(a)

(b)

(c)

(d)