Nephroprotective Effects of Polydatin against Ischemia/Reperfusion Injury: A Role for the PI3K/Akt Signal Pathway

Figure 2

PI3K/Akt pathway participated in the nephroprotective effects of polydatin in renal I/R injury. (a) Western blot for P-Akt and T-Akt proteins in kidneys of mice at 3 d after I/R with or without polydatin and wortmannin treatment. β-actin was used as a control; (b) blood urea nitrogen (BUN), (c) serum creatinine (Scr), (d) histological score of kidney injuries (HSK), (e) renal PCNA expression, (f) renal TUNEL-apoptosis were detected in mice at 3 d after I/R with or without 40 mg/kg of polydatin and 1 mg/kg wortmannin treatment. versus sham; versus vehicle; versus control; (g) P-Akt and T-Akt protein levels in cells with or without OGD/R, in presence or absence of polydatin (10, 20, and 40 μM) and wortmannin (1 μM). (h) In vitro survival analysis of human proximal tubular epithelial HK-2 cells treated with or without polydatin (20 μM) and wortmannin (1 μM) in basal conditions and after OGD/R. Bar graph described from the FACS-based Annexin V/propidium iodide apoptosis assay. versus normoxia; versus OGD; versus polydatin; versus control.

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