Nephroprotective Effects of Polydatin against Ischemia/Reperfusion Injury: A Role for the PI3K/Akt Signal Pathway

Figure 3

PI3K/Akt pathway was involved in polydatin-attenuated expression of the proinflammatory factors. (a) RT-PCR was used for the analysis of TNF-α, IL-1β, COX-2, and iNOS mRNA levels in kidneys of mice with or without polydatin-H (40 mg/kg) and wortmannin (1 mg/kg) at 3 d after I/R. GAPDH was used as a control. (b) ELISA was used to detect the protein levels of TNF-α and IL-1β in renal tissues in mice at 3 d after I/R administrated with different doses of polydatin, with or without wortmannin (1 mg/kg), intraperitoneally. (c) Western blot analysis assessed the expression of COX-2 and iNOS in kidneys of mice at 3 d after I/R with different doses of polydatin treatment. β-actin was used as a control. (d) Western blot analysis was used to detect the expression of COX-2 and iNOS in kidneys of mice at 3 d after I/R with polydatin-H treatment in presence or absence of wortmannin (1 mg/kg). β-actin was used as a control. (e) ELISA was used to detect the levels of PGE-2 and NO in renal tissues in mice at 3 d after I/R administrated with different doses of polydatin, with or without wortmannin (1 mg/kg), intraperitoneally. versus sham; versus vehicle; versus polydatin (10 mg/kg); versus polydatin (20 mg/kg). versus control.

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