Long Term Exposure to Polyphenols of Artichoke (Cynara scolymus L.) Exerts Induction of Senescence Driven Growth Arrest in the MDA-MB231 Human Breast Cancer Cell Line

Figure 1

Low doses of AEs suppress breast cancer cell growth via a caspases-independent mechanism. (a) Cell growth inhibition by AEs. MDA-MB231 cells were treated with increasing concentrations of AEs (from 2.5 to 60 μM) for 10 days. The results are the mean ± SD of at least three independent experiments. Significant statistical differences are indicated by asterisks: . (b) Effects of AEs on caspases pathway. The cells were treated with low doses of AEs (10 and 30 μM) for 10 days or with a high concentration of AEs (400 μM) for 24 h, as a positive control. Whole cell lysates (25 μg/lane) were tested for activation and cleavage of caspase-9. β-Actin was used as a protein loading control.

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