Long Term Exposure to Polyphenols of Artichoke (<i>Cynara scolymus</i> L.) Exerts Induction of Senescence Driven Growth Arrest in the MDA-MB231 Human Breast Cancer Cell Line

<div>Effect of AEs on global DNA methylation levels in MDA-MB231 cells. Low doses of AEs (2.5–30 <i>μ</i>M) for 10 days treatment decreased levels of 5-mC, 5-methylcytosine, in a dose-dependent manner. 5-mC specific antibody was used for (a) and (b) cytostaining and (c) for dot blot analysis on genomic DNA. (a) The image shown is representative of at least three independent experiments. Scale bar: 100 <i>μ</i>m. (b) The 5-methylcytosine positive cells versus total cells were counted by using an inverted microscope (20x) and the results are reported as mean ± SD. (c) The intensity of individual DNA dots shown was quantified by densitometry.</div>

Oxidative Medicine and Cellular Longevity

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Figure 4

Figure 4: Long Term Exposure to Polyphenols of Artichoke (<i>Cynara scolymus</i> L.) Exerts Induction of Senescence Driven Growth Arrest in the MDA-MB231 Human Breast Cancer Cell Line 

Figure 4 | Long Term Exposure to Polyphenols of Artichoke (Cynara scolymus L.) Exerts Induction of Senescence Driven Growth Arrest in the MDA-MB231 Human Breast Cancer Cell Line 