Long Term Exposure to Polyphenols of Artichoke (Cynara scolymus L.) Exerts Induction of Senescence Driven Growth Arrest in the MDA-MB231 Human Breast Cancer Cell Line

Figure 6

ROS production in MDA-MB231 cells treated with AEs. (a) The presence of ROS was detected by DHE fluorescent staining after 10 days treatment. Scale bar: 50 μm, original magnification 20x. (b) Red fluorescent-stained cells versus total cells were counted using an inverted fluorescence microscope. The results are the mean ± SD of at least three independent experiments. Significant statistical differences are indicated by asterisks: . (c) NAC reduced the antiproliferative effect of AEs (30 μM for 10 days). The results are the mean ± SD of at least three independent experiments. Significant statistical differences are indicated by asterisks: .

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