Reactive Oxygen Species in Planarian Regeneration: An Upstream Necessity for Correct Patterning and Brain Formation

Figure 5

DPI exposure does not affect stem cell proliferation. (a) Cell proliferation in DPI-exposed (2 μM) head, trunk, and tail fragments at 4, 48, and 72 hours post amputation. No significant effects of DPI exposure on cell proliferation were observed (). (b) DPI exposure did not affect stem cell proliferation in intact animals. 7 days of DPI exposure before immunostaining (). (c) Schematic figure of the cell cycle and visualization of the different stem cell populations. (d) FACS data of the three stem cell populations (, , and ) during DPI exposure in comparison with control animals at 4 HPA and 72 HPA. Each sample at 4 HPA existed of 6 regenerating head fragments, while measurements at 72 HPA were performed with 4 animals/sample. No effects of the DPI exposure were observed on any of the different neoblast populations or on their distributions.

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