Reactive Oxygen Species in Planarian Regeneration: An Upstream Necessity for Correct Patterning and Brain Formation

Figure 6

Effects of DPI exposure on neoblast differentiation. (a) Smed-wi1 expression at 3 days post amputation (3 DPA) in head, trunk, and tail fragments. Exposure to DPI (3 μM) did not affect the expression (pattern) of the smedwi-1 gene (). Scale bar: 1 mm. (b) Smedwi-1 expression at 7 days post amputation (7 DPA) in head, trunk, and tail fragments. Exposure to DPI (3 μM) did not affect the expression (pattern) of the smedwi-1 gene (). Scale bar: 1 mm. (c) An increase of SMEDWI-1 positive cells was observed at the wound sites of trunk and tail fragments after exposure to DPI (3 μM) in comparison to the control fragments at 7 days post amputation (7 DPA) (). Scale bar: 500 μm. (d) An increase in cell density was noticeable at the wound site of the DPI-exposed animals after 7 days of regeneration (7 DPA) in comparison to the control animals (). Scale bar: 500 μm. (e) A decrease of early neoblast progeny, marked by the expression of smed-NB21.11e, was observed in DPI-exposed (3 μM) trunk and tail fragments (). Scale bar: 500 μm. (f) Quantification of the number of smed-NB.21.11e-positive cells. The number of cells was counted in the prepharyngeal area of the regenerating trunk and tail fragments. A significant decrease was observed in the DPI-exposed trunk and tail fragments in comparison to the trunks and tails of the control groups. . values were obtained via one-way ANOVA analysis.

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