Reactive Oxygen Species in Planarian Regeneration: An Upstream Necessity for Correct Patterning and Brain Formation

Figure 7

DPI exposure impairs proper neuroregeneration. (a) Regenerating head, trunk, and tail fragments exposed to either control medium, 0.01% DMSO, or DPI (2 μM) during a regeneration period of 5 days were stained using anti-SYNORF-1, visualizing the central nervous system (CNS). In the DPI-exposed trunks (4/4) and tails (4/4), the amputated brain failed to regenerate properly, indicated by red arrow heads. (). Close-ups of the blastemas were presented below the respective figure for the regenerating trunk and tail fragments. Scale bars: 1 mm. Scale bars close-ups: 500 μm. (b) Gpas expression in 7-days-regenerating head, trunk, and tail fragments visualized using in situ hybridization. Reduction of the cephalic ganglia in DPI-exposed trunks (4/5) and tails (5/5) was observed, as well as improper formation of the regenerating pharynx in regenerating head pieces (4/4). Close-ups of the blastemas are presented below the respective picture of regenerating trunk and tail fragments. Scale bars: 1 mm. Scale bars close-ups: 500 μm.

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