Thiosemicarbazone p-Substituted Acetophenone Derivatives Promote the Loss of Mitochondrial , GSH Depletion, and Death in K562 Cells

Figure 4

Depletion of GSH in K562 cells by the indicated TSC compounds. GSH levels in K562 cells were determined based on fluorescence after 15 min incubation with o-phthalaldehyde (1 mg/mL). The fluorescence measurements were determined using the 350/420-nm excitation/emission wavelength pair in an F-2500 fluorescence spectrophotometer, as described in Section 2.

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