Research Article

Impact of Atmospheric Microparticles on the Development of Oxidative Stress in Healthy City/Industrial Seaport Residents

Table 2

Analyzed blood parameters and used reagents.

ParameterFunctionsReagent

Thioredoxin 1 (TRX 1)Thioredoxin acts as antioxidant by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange and also serves as electron donor for enzymes such as ribonucleotide reductases, thioredoxin peroxidases (peroxiredoxins), and methionine sulfoxide reductases. It is critical for redox regulation of protein function and signaling via thiol redox control.Thioredoxin-1 ELISA (Northwest Life Science Specialties, LLC, USA)

Thioredoxin reductase 1 (TrxR 1)Thioredoxin reductases are the only enzymes known to catalyze the reduction of thioredoxin.Thioredoxin Reductase 1 ELISA (Northwest Life Science Specialties, LLC, USA)

Malondialdehyde (MDA)Malondialdehyde is a product of lipid peroxidation and prostaglandin biosynthesis. It reacts with DNA to form adducts to deoxyguanosine and deoxyadenosine.Malondialdehyde Assay (Northwest Life Science Specialties, LLC, USA)

8-Hydroxy-2′-deoxyguanosine (8-OHdG)8-OHdG is one of the most sensitive biomarkers for oxidative stress. It is formed when DNA is oxidatively damaged by ROS.8-OHdG ELISA (Northwest Life Science Specialties, LLC, USA)

Protein carbonyl (PC)Protein CO groups are biomarkers of oxidative stress. They are forming relatively early and have relative stability in comparison with other oxidation products.Protein Carbonyl ELISA (BioCell Corporation Ltd., New Zealand)

Antioxidant activity (AOA)Antioxidant activity, or total antioxidant status, is an assessment of the integrated antioxidant system which encompasses all biological components with antioxidant activity.Total antioxidant assay, Total Antioxidant Status Control (Randox Laboratories Ltd., UK)

Glutathione (GSH)Glutathione is the major endogenous antioxidant, participating directly in the neutralization of free radicals and reactive oxygen compounds, as well as maintaining exogenous antioxidants such as vitamins C and E.Glutathione ELISA Kit (MyBioSource, Inc., USA)

Glutathione reductase (GR)Glutathione reductase catalyzes the reduction of glutathione disulfide to the sulfhydryl form glutathione. Their ration is a key factor in properly maintaining the oxidative balance of the cell. Reduced glutathione reduces the oxidized form of the enzyme glutathione peroxidase.Glutathione Reductase ELISA Kit (MyBioSource, Inc., USA)

Glutathione peroxidase (GPx)Glutathione peroxidase reduces lipid hydroperoxides to their corresponding alcohols and free hydrogen peroxide to water.Glutathione Peroxidase 1 ELISA Kit (MyBioSource, Inc., USA)

Superoxide dismutase (CuZn-SOD)Superoxide dismutase converts naturally occurring superoxide radicals to molecular oxygen and hydrogen peroxide. Human Cu/ZnSOD ELISA (eBioscience, USA)

Mitochondrial membrane potential in leukocytes (MMPL)The evaluation of MMPL allows us to estimate cellular energy state.MitoProbe JC-1 Assay Kit (Life Technologies, USA)