Oxidative Stress in Dilated Cardiomyopathy Caused by MYBPC3 Mutation
Increased oxidative stress in the hearts of DCM animals and cardiomyopathy patients. (a) Representative EPR spectra of measured at room temperature. Heart tissues (50 mg) were treated with CMH (1 mM) in Krebs buffer containing deferoxamine mesylate (25 μM) and incubated at 37°C in a water bath for 30 minutes. The reactive oxygen species oxidize CMH into . Blank samples are in the absence of heart tissue. (b) Summary data of ( hearts, in DCM versus WT by Student’s -test, and blank shown for representation). No significant accumulation of was observed in the absence of heart tissues. (c) Comparison of GSH/GSSG ratios ( hearts), (d) carbonyl content ( hearts), and (e) MDA and HAE content ( hearts) in WT and DCM mouse heart tissue homogenates (). (f) Comparison of GSH/GSSG ratios, (g) carbonyl content, and (h) MDA and HAE content in donor ( hearts) and cardiomyopathy ( hearts) human heart tissue homogenates (). Data are represented as mean ± SEM. Significant differences () between mean values were determined using Student’s -test.