Plant Natural Products Calycosin and Gallic Acid Synergistically Attenuate Neutrophil Infiltration and Subsequent Injury in Isoproterenol-Induced Myocardial Infarction: A Possible Role for Leukotriene B4 12-Hydroxydehydrogenase?

Figure 2

Verification of purified calycosin fraction and commercial compounds (i.e., calycosin and formononetin) for LTB4DH induction in human neutrophils. (a) LTB4DH induction by purified calycosin fraction and gallic acid (GA) in combination. Human neutrophils were freshly isolated and treated with purified calycosin fraction (28 μg/mL) and gallic acid (GA) (8 μg/mL) in combination for indicated time points. (b) LTB4DH induction by commercial compounds (i.e., calycosin (CA) and formononetin (FM)) and gallic acid (GA) in combination. Human neutrophils were treated with calycosin (10 μg/mL), formononetin (10 μg/mL), and gallic acid (8 μg/mL), alone or in combination, for 24 h. LTB4DH expression was assayed by RT-PCR. The mean values of three independent experiments were shown. Significance analysis was performed by two-tail paired Student’s t-test. (sample versus untreated control).

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