Plant Natural Products Calycosin and Gallic Acid Synergistically Attenuate Neutrophil Infiltration and Subsequent Injury in Isoproterenol-Induced Myocardial Infarction: A Possible Role for Leukotriene B4 12-Hydroxydehydrogenase?

Figure 3

Suppression of LTB4-induced neutrophils survival by calycosin and gallic acid. (a) Human neutrophils were freshly prepared and were treated with 100 nM LTB4 alone or in combination with calycosin (CA) (10 μg/mL) and gallic acid (GA) (8 μg/mL) for the indicated times. The apoptotic cells were detected by staining Annexin V-FITC/PI and profiled by flow cytometry. The apoptotic cells were characterized into early apoptotic population (lower right panel) and later apoptotic population (upper right panel). (b) Quantitation of neutrophil populations (viable, early apoptotic, and late apoptotic).

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