Plant Natural Products Calycosin and Gallic Acid Synergistically Attenuate Neutrophil Infiltration and Subsequent Injury in Isoproterenol-Induced Myocardial Infarction: A Possible Role for Leukotriene B4 12-Hydroxydehydrogenase?

Figure 4

Synergistic inhibitory effect of gallic acid and calycosin on LTB4-induced chemotaxis of neutrophils. (a) Schematic representation of neutrophil chemotaxis assay. (b) Quantitation of neutrophil chemotaxis. Human neutrophils were Isolated and treated with gallic acid and calycosin, individually or in combination, at 37°C for 24 h. At the end of treatment, neutrophils were loaded with fluorescent probe Calcein AM. Chemotaxis of neutrophils from the upper chamber towards LTB4 in lower chamber was assayed as outlined in (a). Migrated neutrophils were quantitated by measuring the fluorescence intensity on a Zeiss fluorescence spectroscopy (Jena, Germany). Data were expressed as the mean ± SD. Statistical analysis was performed using one-way ANOVA followed by Tukey’s test. (Drugs + LTB versus LTB4).

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