Naphthoquinone Derivative PPE8 Induces Endoplasmic Reticulum Stress in p53 Null H1299 Cells

Figure 2

(a) A549 and H1299 cells were seeded on coverslips overnight. The cells were treated without or with indicated concentrations of PPE8 for 4 h. Cells were fixed and immunostained with ER-specific dye (green) and DAPI (blue). After 5 μM PPE8 treatment, the structures near nuclei could not be observed compared with control cells (arrows) in bright-field microscope. (b) The average green area of each cell was measured (described in methods) without or with indicated concentrations of PPE8 treatment. The average of ER area in cells without PPE8 treatment was expressed as control. Bars represented mean ± SD and expressed as a fold increase from control. Similar results were obtained in three independent experiments.

(a)

(b)