Naphthoquinone Derivative PPE8 Induces Endoplasmic Reticulum Stress in p53 Null H1299 Cells

Figure 6

(a) H1299 and A549 cells were treated without or with etoposide (100 μg/mL) or 5 μM PPE8 treatment for 8 h. (b) Before PPE8 treatment, A549 cells were transfected with control siRNA or p53 siRNA for 24 h. The cell lysates were collected from H1299 and A549 cells and immunoblotted with p53, p21, GRP78, p-JNK, JNK, and β-actin antibodies. (c) Quantification of GRP78 of 3 independent experiments. The signal detected in untreated cells was taken as control of each experiment. Bars represented mean ± SD and expressed as a fold increase from control. Asterisks indicate the values that are significantly different from corresponding control ().

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