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Figure 2: Antioxidative treatment completely reverses HFD-induced ROS production in kidneys and partially reverses oxidative stress in the circulation. Mice were fed either a normal diet, a high-fat diet (HFD), or a HFD supplemented with corabion for eight weeks starting at an age of five weeks. (a) Production of ROS as measure of oxidative stress was determined in 10 μm kidney cryosections by DHE assays at the indicated times. Red fluorescence of ethidium in the nuclei is depicted. Bar = 50 μm. (b) Levels of tetrahydrobiopterin (BH4) (c) and dihydrobiopterin (BH2) in plasma samples as measure of vascular oxidative stress were determined by capillary electrophoresis at the indicated times. The -axes indicate weeks after start of the respective diets. ; ; (significance control versus HFD); ; (significance HFD versus HFD + corabion).