Antioxidative Diet Supplementation Reverses High-Fat Diet-Induced Increases of Cardiovascular Risk Factors in Mice

Figure 4

Antioxidative treatment partially reverses early signs of microinflammation after two weeks. Mice were fed either a control diet (ctrl), a high-fat diet (HFD), or a HFD with corabion for two weeks starting at an age of five weeks. (a) Production of IL6 and TNF mRNAs as measure of an early inflammatory response was determined by semiquantitative RT-PCR of cDNA derived from kidney of the respective mice.   β-actin and GAPDH were used as housekeeping gene. Representative images of triplicates from two animals per group are shown. (b) Levels of C-reactive protein (CRP) in blood serum samples, as independent inflammatory marker in the circulation, were determined by agglutination. Depicted are changes in percent compared to mice on control diet (ctrl set to 100%). measured in the triplicates; .

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