In Vitro Toxicity of Epigallocatechin Gallate in Rat Liver Mitochondria and Hepatocytes

Table 2

EGCG-induced changes in mitochondrial respiration. The consumption of oxygen is expressed in %, where 100% is oxygen consumption of control hepatocytes at state 4 for given experiment. Other values are recounted to this value for given experiment and respiratory control ratio is calculated. G + M, glutamate + malate, ADP, adenosine diphosphate, Cyt c, cytochrome c, Rot, rotenone, SUC, succinate, and RCR I, respiratory control ratio of complex I. ( = 5-6) *, **, and *** versus medium-treated controls; , 0.01, and 0.001, respectively.

Substrates added	EGCG concentration (μmol/L)
	0 (control)	10	50	100	200
G + M	100	108.8 ± 8.8	115.2 ± 15.9	114.1 ± 13.7	134.9 ± 10.3^***
ADP	561.1 ± 129.4	623.9 ± 125.8	481.6 ± 48.0	506.8 ± 117.6	452.6 ± 136.4
Cyt c	561.2 ± 129.8	648.4 ± 115.0	598.8 ± 89.0	750.9 ± 158.8	842.9 ± 189.0^*
Rot	61.4 ± 14.2	76.1 ± 7.9	177.2 ± 55.3^*	299.3 ± 72.8^***	446.0 ± 106.8^***
SUC	1191.6 ± 25.4	1203.4 ± 122.8	1282.7 ± 78.8	1217.4 ± 53.7	1104.0 ± 146.7
RCR I	5.7 ± 1.1	5.7 ± 1.0	4.2 ± 0.7	4.5 ± 1.4	3.3 ± 0.8^**